runx 2  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Hippo/YAP1 Signaling Regulates the Oligodendrocyte–Astrocyte Fate Switch and Ependymal Gene Expression in Adult Spinal Cord Stem Cells

doi: 10.1101/2025.10.01.677488

Figure Lengend Snippet: Transcriptomic and proteomic profiles of PDGFRA-GFP⁺ and GFP⁻ spinal cord neurosphere cells A. Volcano plot of bulk RNA-seq comparing GFP⁺ and GFP⁻ cells under proliferative conditions (n = 3). Differentially expressed genes were defined as those with log 2 (|fold change| > 1 and q-value < 0.05. B. GSEA (Gene Set Enrichment Analysis) of bulk RNA-seq. Using an established OPC gene list, GFP⁺ cells show strong enrichment with reciprocal depletion in GFP⁻ cells, whereas GFP⁻ cells are enriched for radial glia, astrocyte, and ependymal markers. C. Heatmap (linear fold change) of selected differentially expressed genes between GFP⁺ vs GFP⁻ cells (p < 0.05). GFP⁺ cells show enriched expression of OPC markers (e.g., Gpr17, Pdgfra, Cspg4, Ascl1, Olig2) , while GFP⁻ cells are enriched for astrocytic markers (e.g., Gfap, Hopx, Sparc, Fabp7 ) D–E. Bubble charts of selected pathways from Table S1 significantly enriched in GFP⁺ and GFP⁻ cells. Red arrows in panel E highlight enrichment of Hippo/YAP1 signaling components in GFP⁻ cells. F. Volcano plots of proteomic profiling of GFP⁺ versus GFP⁻ cells under proliferative and differentiative conditions. Differentially expressed proteins were defined using thresholds of |linear fold change| > 1.2 (i.e., |log₂ fold change| > 0.26) and q-value < 0.05 (n = 5). G. Scatter plot showing correlation between RNA-seq and proteomic fold changes, demonstrating positive concordance between datasets (Pearson r = 0.65, p < 0.001). H. Heatmap (linear fold change) of selected differentially expressed proteins (p < 0.05) between GFP⁺ and GFP⁻ cells. Proteins representative of the oligodendrocyte lineage are shown in red/purple and those of the astrocytic lineage in blue. Linear fold changes (GFP⁺ vs GFP⁻) are indicated in yellow for each protein.

Article Snippet: Pharmacological activation of the Hippo/YAP1 pathway was performed by adding 5 μM TDI-011536 (MedChemExpress) to the culture medium on days 0 and 3.

Techniques: RNA Sequencing, Expressing